Normalization of circulating microRNA expression data obtained by quantitative real-time RT-PCR
نویسندگان
چکیده
منابع مشابه
Normalization of circulating microRNA expression data obtained by quantitative real-time RT-PCR
The high-throughput analysis of microRNAs (miRNAs) circulating within the blood of healthy and diseased individuals is an active area of biomarker research. Whereas quantitative real-time reverse transcription polymerase chain reaction (qPCR)-based methods are widely used, it is yet unresolved how the data should be normalized. Here, we show that a combination of different algorithms results in...
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Quantitative real-time PCR represents a highly sensitive and powerful technique for the quantitation of nucleic acids. It has a tremendous potential for the high-throughput analysis of gene expression in research and routine diagnostics. However, the major hurdle is not the practical performance of the experiments themselves but rather the efficient evaluation and the mathematical and statistic...
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SUMMARY Q-Gene is an application for the processing of quantitative real-time RT-PCR data. It offers the user the possibility to freely choose between two principally different procedures to calculate normalized gene expressions as either means of Normalized Expressions or Mean Normalized Expressions. In this contribution it will be shown that the calculation of Mean Normalized Expressions has ...
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ژورنال
عنوان ژورنال: Briefings in Bioinformatics
سال: 2015
ISSN: 1467-5463,1477-4054
DOI: 10.1093/bib/bbv056